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dc.contributor.authorBorchert, Erik
dc.contributor.authorGarcia-Moyano, Antonio
dc.contributor.authorSánchez-Carrillo, Sergio
dc.contributor.authorDahlgren, Thomas Gunnar
dc.contributor.authorSlaby, B
dc.contributor.authorBjerga, Gro Elin Kjæreng
dc.contributor.authorFerrer, Manuel
dc.contributor.authorFranzenburg, Sören
dc.contributor.authorHentschel, Ute
dc.date.accessioned2021-10-13T13:07:37Z
dc.date.available2021-10-13T13:07:37Z
dc.date.created2021-04-16T14:15:54Z
dc.date.issued2021
dc.identifier.citationmSystems. 2021, 6 (1), 1-19.
dc.identifier.issn2379-5077
dc.identifier.urihttps://hdl.handle.net/11250/2799833
dc.description.abstractThe marine bone biome is a complex assemblage of macro- and microorganisms; however, the enzymatic repertoire to access bone-derived nutrients remains unknown. The bone matrix is a composite material made up mainly of organic collagen and inorganic hydroxyapatite. We conducted field experiments to study microbial assemblages that can use organic bone components as nutrient source. Bovine and turkey bones were deposited at 69 m depth in a Norwegian fjord (Byfjorden, Bergen). Metagenomic sequence analysis was used to assess the functional potential of microbial assemblages from bone surface and the bone-eating worm Osedax mucofloris, which is a frequent colonizer of whale falls and known to degrade bone. The bone microbiome displayed a surprising taxonomic diversity revealed by the examination of 59 high-quality metagenome-assembled genomes from at least 23 bacterial families. Over 700 genes encoding enzymes from 12 relevant enzymatic families pertaining to collagenases, peptidases, and glycosidases putatively involved in bone degradation were identified. Metagenome-assembled genomes (MAGs) of the class Bacteroidia contained the most diverse gene repertoires. We postulate that demineralization of inorganic bone components is achieved by a timely succession of a closed sulfur biogeochemical cycle between sulfur-oxidizing and sulfur-reducing bacteria, causing a drop in pH and subsequent enzymatic processing of organic components in the bone surface communities. An unusually large and novel collagen utilization gene cluster was retrieved from one genome belonging to the gammaproteobacterial genus Colwellia.
dc.language.isoeng
dc.rightsCC BY 4.0
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleDeciphering a Marine Bone-Degrading Microbiome Reveals a Complex Community Effort
dc.typePeer reviewed
dc.typeJournal article
dc.description.versionpublishedVersion
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.1128/mSystems.01218-20
dc.identifier.cristin1904619
dc.source.journalmSystems
dc.source.volume6
dc.source.issue1
dc.source.pagenumber1-19


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CC BY 4.0
Except where otherwise noted, this item's license is described as CC BY 4.0