Vis enkel innførsel

dc.contributor.authorIslam, Tajul
dc.contributor.authorGessesse, Amare
dc.contributor.authorGarcia-Moyano, Antonio
dc.contributor.authorMurrell, J. Colin
dc.contributor.authorØvreås, Lise
dc.date.accessioned2021-03-29T07:45:56Z
dc.date.available2021-03-29T07:45:56Z
dc.date.created2020-11-09T11:59:05Z
dc.date.issued2020
dc.identifier.citationMicroorganisms. 2020, 8 (2), 1-18.en_US
dc.identifier.issn2076-2607
dc.identifier.urihttps://hdl.handle.net/11250/2735836
dc.description.abstractAerobic moderately thermophilic and thermophilic methane-oxidizing bacteria make a substantial contribution in the control of global warming through biological reduction of methane emissions and have a unique capability of utilizing methane as their sole carbon and energy source. Here, we report a novel moderately thermophilic Methylococcus-like Type Ib methanotroph recovered from an alkaline thermal spring (55.4 °C and pH 8.82) in the Ethiopian Rift Valley. The isolate, designated LS7-MC, most probably represents a novel species of a new genus in the family Methylococcaceae of the class Gammaproteobacteria. The 16S rRNA gene phylogeny indicated that strain LS7-MC is distantly related to the closest described relative, Methylococcus capsulatus (92.7% sequence identity). Growth was observed at temperatures of 30–60 °C (optimal, 51–55 °C), and the cells possessed Type I intracellular membrane (ICM). The comparison of the pmoA gene sequences showed that the strain was most closely related to M. capsulatus (87.8%). Soluble methane monooxygenase (sMMO) was not detected, signifying the biological oxidation process from methane to methanol by the particulate methane monooxygenase (pMMO). The other functional genes mxaF, cbbL and nifH were detected by PCR. To our knowledge, the new strain is the first isolated moderately thermophilic methanotroph from an alkaline thermal spring of the family Methylococcaceae. Furthermore, LS7-MC represents a previously unrecognized biological methane sink in thermal habitats, expanding our knowledge of its ecological role in methane cycling and aerobic methanotrophy.en_US
dc.language.isoengen_US
dc.rightsNavngivelse 4.0 Internasjonal*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/deed.no*
dc.titleA Novel Moderately Thermophilic Type Ib Methanotroph Isolated from an Alkaline Thermal Spring in the Ethiopian Rift Valleyen_US
dc.typePeer revieweden_US
dc.typeJournal articleen_US
dc.rights.holder© 2020, Authors
dc.description.versionpublishedVersionen_US
cristin.ispublishedtrue
cristin.fulltextoriginal
cristin.qualitycode1
dc.identifier.doi10.3390/microorganisms8020250
dc.identifier.cristin1846102
dc.source.journalMicroorganismsen_US
dc.source.volume8en_US
dc.source.issue2en_US
dc.source.pagenumber1-18en_US


Tilhørende fil(er)

Thumbnail

Denne innførselen finnes i følgende samling(er)

Vis enkel innførsel

Navngivelse 4.0 Internasjonal
Med mindre annet er angitt, så er denne innførselen lisensiert som Navngivelse 4.0 Internasjonal